<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with the Workiva Platform-->
<!--Copyright 2025 Workiva-->
<!--r:1708f329-0981-496d-9beb-35bd936a65ba,g:1f2b8fa6-b869-469b-9207-cce487a1c6a0-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:roleRef roleURI="http://www.humacyte.com/role/ConsolidatedBalanceSheets" xlink:type="simple" xlink:href="huma-20241231.xsd#ConsolidatedBalanceSheets"/>
  <link:calculationLink xlink:role="http://www.humacyte.com/role/ConsolidatedBalanceSheets" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesAndStockholdersEquity_8ffea3a0-f8f9-4ebb-a127-35950335c9e9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LiabilitiesAndStockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Liabilities_38800909-773a-4814-8189-e4f85fb67683" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Liabilities"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_8ffea3a0-f8f9-4ebb-a127-35950335c9e9" xlink:to="loc_us-gaap_Liabilities_38800909-773a-4814-8189-e4f85fb67683" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommitmentsAndContingencies_65468a07-744e-4e09-afa5-9904e5a5713d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommitmentsAndContingencies"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_8ffea3a0-f8f9-4ebb-a127-35950335c9e9" xlink:to="loc_us-gaap_CommitmentsAndContingencies_65468a07-744e-4e09-afa5-9904e5a5713d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_1041dc18-934e-4327-b8fa-1a0dcdb7e16b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockholdersEquity"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_8ffea3a0-f8f9-4ebb-a127-35950335c9e9" xlink:to="loc_us-gaap_StockholdersEquity_1041dc18-934e-4327-b8fa-1a0dcdb7e16b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Assets_a6cb0c0a-00cd-4d23-af8d-d8427142394e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Assets"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsNoncurrent_d1c01bd2-6cd9-4b50-bca3-4135cbc1745b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherAssetsNoncurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_a6cb0c0a-00cd-4d23-af8d-d8427142394e" xlink:to="loc_us-gaap_OtherAssetsNoncurrent_d1c01bd2-6cd9-4b50-bca3-4135cbc1745b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_89750603-b7d1-4406-8480-1b69c87a245f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_a6cb0c0a-00cd-4d23-af8d-d8427142394e" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentNet_89750603-b7d1-4406-8480-1b69c87a245f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCashNoncurrent_c8d58893-6a3c-4c03-9055-b6bfec5cfa56" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RestrictedCashNoncurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_a6cb0c0a-00cd-4d23-af8d-d8427142394e" xlink:to="loc_us-gaap_RestrictedCashNoncurrent_c8d58893-6a3c-4c03-9055-b6bfec5cfa56" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinanceLeaseRightOfUseAsset_2cecb592-c90c-4b1b-a80e-3106b5aefa30" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FinanceLeaseRightOfUseAsset"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_a6cb0c0a-00cd-4d23-af8d-d8427142394e" xlink:to="loc_us-gaap_FinanceLeaseRightOfUseAsset_2cecb592-c90c-4b1b-a80e-3106b5aefa30" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsCurrent_2a927576-0186-4a57-a494-3b2cdce53d02" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AssetsCurrent"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_a6cb0c0a-00cd-4d23-af8d-d8427142394e" xlink:to="loc_us-gaap_AssetsCurrent_2a927576-0186-4a57-a494-3b2cdce53d02" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_0e7cea46-581e-494a-8bfe-d94bfcfd64e2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockValue_3026cb05-8e9f-49bb-92a2-f8fb16867179" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockValue"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_0e7cea46-581e-494a-8bfe-d94bfcfd64e2" xlink:to="loc_us-gaap_PreferredStockValue_3026cb05-8e9f-49bb-92a2-f8fb16867179" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockValue_1379fdfd-2b75-4496-8e14-73bd1e425868" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockValue"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_0e7cea46-581e-494a-8bfe-d94bfcfd64e2" xlink:to="loc_us-gaap_CommonStockValue_1379fdfd-2b75-4496-8e14-73bd1e425868" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapital_1f402767-4b4e-4ff6-9a3f-3be1a28a0e00" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AdditionalPaidInCapital"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_0e7cea46-581e-494a-8bfe-d94bfcfd64e2" xlink:to="loc_us-gaap_AdditionalPaidInCapital_1f402767-4b4e-4ff6-9a3f-3be1a28a0e00" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsAccumulatedDeficit_852aec26-193d-4b22-b002-149256f91798" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RetainedEarningsAccumulatedDeficit"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_0e7cea46-581e-494a-8bfe-d94bfcfd64e2" xlink:to="loc_us-gaap_RetainedEarningsAccumulatedDeficit_852aec26-193d-4b22-b002-149256f91798" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Liabilities_d3d8c01a-1574-4829-a4a9-9b78dd71c706" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Liabilities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UnsecuredLongTermDebt_022749f1-01ee-4cda-9b0d-b03c8caa54be" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_UnsecuredLongTermDebt"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_d3d8c01a-1574-4829-a4a9-9b78dd71c706" xlink:to="loc_us-gaap_UnsecuredLongTermDebt_022749f1-01ee-4cda-9b0d-b03c8caa54be" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinanceLeaseLiabilityNoncurrent_ea4f8232-82f5-4727-832c-eda10fb57b20" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FinanceLeaseLiabilityNoncurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_d3d8c01a-1574-4829-a4a9-9b78dd71c706" xlink:to="loc_us-gaap_FinanceLeaseLiabilityNoncurrent_ea4f8232-82f5-4727-832c-eda10fb57b20" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesCurrent_83560252-eba9-4221-a54c-c703757cc433" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LiabilitiesCurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_d3d8c01a-1574-4829-a4a9-9b78dd71c706" xlink:to="loc_us-gaap_LiabilitiesCurrent_83560252-eba9-4221-a54c-c703757cc433" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WarrantsAndRightsOutstanding_f3889823-dce8-40d4-98ee-64aa722ea3fb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_WarrantsAndRightsOutstanding"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_d3d8c01a-1574-4829-a4a9-9b78dd71c706" xlink:to="loc_us-gaap_WarrantsAndRightsOutstanding_f3889823-dce8-40d4-98ee-64aa722ea3fb" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeLiabilitiesNoncurrent_33b84dc5-45d2-4d89-803d-8e2036c52eae" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DerivativeLiabilitiesNoncurrent"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_d3d8c01a-1574-4829-a4a9-9b78dd71c706" xlink:to="loc_us-gaap_DerivativeLiabilitiesNoncurrent_33b84dc5-45d2-4d89-803d-8e2036c52eae" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationContingentConsiderationLiabilityNoncurrent_a3729328-8d43-4017-97ad-9fe75c7013cd" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationContingentConsiderationLiabilityNoncurrent"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_d3d8c01a-1574-4829-a4a9-9b78dd71c706" xlink:to="loc_us-gaap_BusinessCombinationContingentConsiderationLiabilityNoncurrent_a3729328-8d43-4017-97ad-9fe75c7013cd" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherLiabilitiesNoncurrent_2d82d5ff-283f-4781-8413-5d33fc7f32b3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherLiabilitiesNoncurrent"/>
    <link:calculationArc order="7" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_d3d8c01a-1574-4829-a4a9-9b78dd71c706" xlink:to="loc_us-gaap_OtherLiabilitiesNoncurrent_2d82d5ff-283f-4781-8413-5d33fc7f32b3" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesCurrent_0ac49422-da40-4ece-9389-54a1447fe509" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LiabilitiesCurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsPayableCurrent_8e06f647-4d56-4895-bd4d-1a2970c601c6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccountsPayableCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_0ac49422-da40-4ece-9389-54a1447fe509" xlink:to="loc_us-gaap_AccountsPayableCurrent_8e06f647-4d56-4895-bd4d-1a2970c601c6" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccruedLiabilitiesCurrent_7c85bb53-1417-48cc-aaad-30314e5e9dd1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccruedLiabilitiesCurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_0ac49422-da40-4ece-9389-54a1447fe509" xlink:to="loc_us-gaap_AccruedLiabilitiesCurrent_7c85bb53-1417-48cc-aaad-30314e5e9dd1" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinanceLeaseLiabilityCurrent_085886cf-f8de-4cf1-8fcc-b12158b74e38" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FinanceLeaseLiabilityCurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_0ac49422-da40-4ece-9389-54a1447fe509" xlink:to="loc_us-gaap_FinanceLeaseLiabilityCurrent_085886cf-f8de-4cf1-8fcc-b12158b74e38" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherLiabilitiesCurrent_8024b1f0-776e-4ab6-bca9-977465cb5440" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherLiabilitiesCurrent"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_0ac49422-da40-4ece-9389-54a1447fe509" xlink:to="loc_us-gaap_OtherLiabilitiesCurrent_8024b1f0-776e-4ab6-bca9-977465cb5440" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsCurrent_fff89b12-ad8d-47d0-9713-a8a61fb94151" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AssetsCurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_78a1f405-ac12-4f31-a3fa-4ec347c9875b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PrepaidExpenseAndOtherAssetsCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_fff89b12-ad8d-47d0-9713-a8a61fb94151" xlink:to="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_78a1f405-ac12-4f31-a3fa-4ec347c9875b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_5dcbb702-568e-44ba-867e-41474e2d6cbf" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_fff89b12-ad8d-47d0-9713-a8a61fb94151" xlink:to="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_5dcbb702-568e-44ba-867e-41474e2d6cbf" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.humacyte.com/role/ConsolidatedStatementsofOperationsandComprehensiveLoss" xlink:type="simple" xlink:href="huma-20241231.xsd#ConsolidatedStatementsofOperationsandComprehensiveLoss"/>
  <link:calculationLink xlink:role="http://www.humacyte.com/role/ConsolidatedStatementsofOperationsandComprehensiveLoss" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_5e47cf2c-0e79-40e3-83cb-55578d1352d7" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingExpenses_4d28c221-2e71-4109-8e2e-5d964676fde5" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingExpenses"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingIncomeLoss_5e47cf2c-0e79-40e3-83cb-55578d1352d7" xlink:to="loc_us-gaap_OperatingExpenses_4d28c221-2e71-4109-8e2e-5d964676fde5" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_d5e96379-de54-45a2-b221-6f18ac0158a0" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetIncomeLoss"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonoperatingIncomeExpense_0bb17e46-1756-4ed2-a35d-0e7e92c256e1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NonoperatingIncomeExpense"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLoss_d5e96379-de54-45a2-b221-6f18ac0158a0" xlink:to="loc_us-gaap_NonoperatingIncomeExpense_0bb17e46-1756-4ed2-a35d-0e7e92c256e1" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_8045b825-4aac-475e-9129-d36f31345b44" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLoss_d5e96379-de54-45a2-b221-6f18ac0158a0" xlink:to="loc_us-gaap_OperatingIncomeLoss_8045b825-4aac-475e-9129-d36f31345b44" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ComprehensiveIncomeNetOfTax_e058a66a-78b1-4c21-9f72-ae12d19b94af" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ComprehensiveIncomeNetOfTax"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_16abde9a-3d21-415c-951c-d7aa86267f8f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_ComprehensiveIncomeNetOfTax_e058a66a-78b1-4c21-9f72-ae12d19b94af" xlink:to="loc_us-gaap_OperatingIncomeLoss_16abde9a-3d21-415c-951c-d7aa86267f8f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonoperatingIncomeExpense_9bec3824-b3b8-41d8-a644-9309d4f33080" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NonoperatingIncomeExpense"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_ComprehensiveIncomeNetOfTax_e058a66a-78b1-4c21-9f72-ae12d19b94af" xlink:to="loc_us-gaap_NonoperatingIncomeExpense_9bec3824-b3b8-41d8-a644-9309d4f33080" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonoperatingIncomeExpense_9c67d1f1-89af-448e-8213-70b0ab1504b5" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NonoperatingIncomeExpense"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpenseNonoperating_9452c80b-b25c-4709-967b-7ec972f3299e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_InterestExpenseNonoperating"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NonoperatingIncomeExpense_9c67d1f1-89af-448e-8213-70b0ab1504b5" xlink:to="loc_us-gaap_InterestExpenseNonoperating_9452c80b-b25c-4709-967b-7ec972f3299e" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentIncomeInterest_d779a8f4-dd3f-4243-bb87-03ed79348ec4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_InvestmentIncomeInterest"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NonoperatingIncomeExpense_9c67d1f1-89af-448e-8213-70b0ab1504b5" xlink:to="loc_us-gaap_InvestmentIncomeInterest_d779a8f4-dd3f-4243-bb87-03ed79348ec4" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationContingentConsiderationArrangementsChangeInAmountOfContingentConsiderationLiability1_70469bb5-af1a-4fe0-a895-8d7cbfba38c0" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationContingentConsiderationArrangementsChangeInAmountOfContingentConsiderationLiability1"/>
    <link:calculationArc order="3" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NonoperatingIncomeExpense_9c67d1f1-89af-448e-8213-70b0ab1504b5" xlink:to="loc_us-gaap_BusinessCombinationContingentConsiderationArrangementsChangeInAmountOfContingentConsiderationLiability1_70469bb5-af1a-4fe0-a895-8d7cbfba38c0" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GainsLossesOnExtinguishmentOfDebt_09d05256-ef4b-47d6-a942-26125fec431a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_GainsLossesOnExtinguishmentOfDebt"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NonoperatingIncomeExpense_9c67d1f1-89af-448e-8213-70b0ab1504b5" xlink:to="loc_us-gaap_GainsLossesOnExtinguishmentOfDebt_09d05256-ef4b-47d6-a942-26125fec431a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_huma_CARESActEmployeeRetentionCredit_4802ade1-bc79-43d9-acf0-12025effe996" xlink:href="huma-20241231.xsd#huma_CARESActEmployeeRetentionCredit"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NonoperatingIncomeExpense_9c67d1f1-89af-448e-8213-70b0ab1504b5" xlink:to="loc_huma_CARESActEmployeeRetentionCredit_4802ade1-bc79-43d9-acf0-12025effe996" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UnrealizedGainLossOnDerivatives_f5589ad7-d7d2-4722-b7f0-a47dc5de030a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_UnrealizedGainLossOnDerivatives"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NonoperatingIncomeExpense_9c67d1f1-89af-448e-8213-70b0ab1504b5" xlink:to="loc_us-gaap_UnrealizedGainLossOnDerivatives_f5589ad7-d7d2-4722-b7f0-a47dc5de030a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingExpenses_fe484021-dfe8-42d1-8036-65d97fa1be0b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingExpenses"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResearchAndDevelopmentExpense_4f3ed9e6-0485-42df-bee5-d948d64953c9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ResearchAndDevelopmentExpense"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingExpenses_fe484021-dfe8-42d1-8036-65d97fa1be0b" xlink:to="loc_us-gaap_ResearchAndDevelopmentExpense_4f3ed9e6-0485-42df-bee5-d948d64953c9" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GeneralAndAdministrativeExpense_34b1bc55-2f6c-474c-8a04-62581bcef913" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_GeneralAndAdministrativeExpense"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingExpenses_fe484021-dfe8-42d1-8036-65d97fa1be0b" xlink:to="loc_us-gaap_GeneralAndAdministrativeExpense_34b1bc55-2f6c-474c-8a04-62581bcef913" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.humacyte.com/role/ConsolidatedStatementsofCashFlows" xlink:type="simple" xlink:href="huma-20241231.xsd#ConsolidatedStatementsofCashFlows"/>
  <link:calculationLink xlink:role="http://www.humacyte.com/role/ConsolidatedStatementsofCashFlows" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_22e41670-5967-4c2f-8092-1fe32491ad39" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromIssuanceOfCommonStock_93ee230f-99d9-451a-bdfb-a81622222de4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProceedsFromIssuanceOfCommonStock"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_22e41670-5967-4c2f-8092-1fe32491ad39" xlink:to="loc_us-gaap_ProceedsFromIssuanceOfCommonStock_93ee230f-99d9-451a-bdfb-a81622222de4" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromIssuanceOfOtherLongTermDebt_f16ea2e6-0c4d-4615-9b33-d444b64de5f7" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProceedsFromIssuanceOfOtherLongTermDebt"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_22e41670-5967-4c2f-8092-1fe32491ad39" xlink:to="loc_us-gaap_ProceedsFromIssuanceOfOtherLongTermDebt_f16ea2e6-0c4d-4615-9b33-d444b64de5f7" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromIssuanceOfUnsecuredDebt_bf5bd146-c54e-4da1-987a-c0c38deb899b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProceedsFromIssuanceOfUnsecuredDebt"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_22e41670-5967-4c2f-8092-1fe32491ad39" xlink:to="loc_us-gaap_ProceedsFromIssuanceOfUnsecuredDebt_bf5bd146-c54e-4da1-987a-c0c38deb899b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsOfStockIssuanceCosts_97c6db20-91a2-41c6-8664-ec075397c645" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PaymentsOfStockIssuanceCosts"/>
    <link:calculationArc order="4" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_22e41670-5967-4c2f-8092-1fe32491ad39" xlink:to="loc_us-gaap_PaymentsOfStockIssuanceCosts_97c6db20-91a2-41c6-8664-ec075397c645" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsOfDebtExtinguishmentCosts_54b91c72-620b-4646-b963-02eb3a70d359" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PaymentsOfDebtExtinguishmentCosts"/>
    <link:calculationArc order="5" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_22e41670-5967-4c2f-8092-1fe32491ad39" xlink:to="loc_us-gaap_PaymentsOfDebtExtinguishmentCosts_54b91c72-620b-4646-b963-02eb3a70d359" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinanceLeasePrincipalPayments_54eda053-f71c-4263-ac19-71492d955952" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FinanceLeasePrincipalPayments"/>
    <link:calculationArc order="6" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_22e41670-5967-4c2f-8092-1fe32491ad39" xlink:to="loc_us-gaap_FinanceLeasePrincipalPayments_54eda053-f71c-4263-ac19-71492d955952" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromStockOptionsExercised_094754b0-ef4e-4abf-92d9-daa0d627bca4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProceedsFromStockOptionsExercised"/>
    <link:calculationArc order="7" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_22e41670-5967-4c2f-8092-1fe32491ad39" xlink:to="loc_us-gaap_ProceedsFromStockOptionsExercised_094754b0-ef4e-4abf-92d9-daa0d627bca4" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RepaymentsOfSecuredDebt_aa9203d2-179f-42ff-8449-1ab4e4647022" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RepaymentsOfSecuredDebt"/>
    <link:calculationArc order="8" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_22e41670-5967-4c2f-8092-1fe32491ad39" xlink:to="loc_us-gaap_RepaymentsOfSecuredDebt_aa9203d2-179f-42ff-8449-1ab4e4647022" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsOfDebtIssuanceCosts_937e076d-6e17-477d-8243-abf3d3cbbf83" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PaymentsOfDebtIssuanceCosts"/>
    <link:calculationArc order="9" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_22e41670-5967-4c2f-8092-1fe32491ad39" xlink:to="loc_us-gaap_PaymentsOfDebtIssuanceCosts_937e076d-6e17-477d-8243-abf3d3cbbf83" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_45e6d6ef-f8ca-4ea1-a0b9-e57ab17bca97" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromMaturitiesPrepaymentsAndCallsOfShorttermInvestments_ee70a16f-c436-4055-b454-58fbbe20924f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProceedsFromMaturitiesPrepaymentsAndCallsOfShorttermInvestments"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_45e6d6ef-f8ca-4ea1-a0b9-e57ab17bca97" xlink:to="loc_us-gaap_ProceedsFromMaturitiesPrepaymentsAndCallsOfShorttermInvestments_ee70a16f-c436-4055-b454-58fbbe20924f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquirePropertyPlantAndEquipment_b075cfbf-260b-464c-9f0f-75ff923ebf82" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PaymentsToAcquirePropertyPlantAndEquipment"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_45e6d6ef-f8ca-4ea1-a0b9-e57ab17bca97" xlink:to="loc_us-gaap_PaymentsToAcquirePropertyPlantAndEquipment_b075cfbf-260b-464c-9f0f-75ff923ebf82" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_2b40e1a1-ac43-4012-b831-60662ebe5dd0" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpenseInterestBearingLiability_e22e5e24-5926-4b55-87cb-a42882876e79" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_InterestExpenseInterestBearingLiability"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_2b40e1a1-ac43-4012-b831-60662ebe5dd0" xlink:to="loc_us-gaap_InterestExpenseInterestBearingLiability_e22e5e24-5926-4b55-87cb-a42882876e79" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_9629bf6f-f215-4518-8efa-280eaa34b215" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetIncomeLoss"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_2b40e1a1-ac43-4012-b831-60662ebe5dd0" xlink:to="loc_us-gaap_NetIncomeLoss_9629bf6f-f215-4518-8efa-280eaa34b215" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensation_75fa575a-09af-409f-9169-13a5530c9b7d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensation"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_2b40e1a1-ac43-4012-b831-60662ebe5dd0" xlink:to="loc_us-gaap_ShareBasedCompensation_75fa575a-09af-409f-9169-13a5530c9b7d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinanceLeaseRightOfUseAssetAmortization_de02f7d0-d3e4-40b0-99cd-98736fcd8aae" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FinanceLeaseRightOfUseAssetAmortization"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_2b40e1a1-ac43-4012-b831-60662ebe5dd0" xlink:to="loc_us-gaap_FinanceLeaseRightOfUseAssetAmortization_de02f7d0-d3e4-40b0-99cd-98736fcd8aae" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GainsLossesOnExtinguishmentOfDebt_9f75713f-0c8e-43b4-be90-b936ddd02490" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_GainsLossesOnExtinguishmentOfDebt"/>
    <link:calculationArc order="5" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_2b40e1a1-ac43-4012-b831-60662ebe5dd0" xlink:to="loc_us-gaap_GainsLossesOnExtinguishmentOfDebt_9f75713f-0c8e-43b4-be90-b936ddd02490" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AmortizationOfDebtDiscountPremium_c761a838-2bb7-412a-b1ec-cf509a305d19" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AmortizationOfDebtDiscountPremium"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_2b40e1a1-ac43-4012-b831-60662ebe5dd0" xlink:to="loc_us-gaap_AmortizationOfDebtDiscountPremium_c761a838-2bb7-412a-b1ec-cf509a305d19" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UnrealizedGainLossOnDerivatives_827aeeec-afd3-4aa7-b632-177963c1c63d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_UnrealizedGainLossOnDerivatives"/>
    <link:calculationArc order="7" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_2b40e1a1-ac43-4012-b831-60662ebe5dd0" xlink:to="loc_us-gaap_UnrealizedGainLossOnDerivatives_827aeeec-afd3-4aa7-b632-177963c1c63d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsReceivable_9de8f7da-1208-4015-a963-1543f88cc713" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInAccountsReceivable"/>
    <link:calculationArc order="8" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_2b40e1a1-ac43-4012-b831-60662ebe5dd0" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsReceivable_9de8f7da-1208-4015-a963-1543f88cc713" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseRightOfUseAssetAmortizationExpense_8f0ca162-1cd4-4ae2-ac28-c4f56b4c5581" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingLeaseRightOfUseAssetAmortizationExpense"/>
    <link:calculationArc order="9" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_2b40e1a1-ac43-4012-b831-60662ebe5dd0" xlink:to="loc_us-gaap_OperatingLeaseRightOfUseAssetAmortizationExpense_8f0ca162-1cd4-4ae2-ac28-c4f56b4c5581" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Depreciation_93aa7a35-796d-402c-8f7a-97a211b712db" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Depreciation"/>
    <link:calculationArc order="10" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_2b40e1a1-ac43-4012-b831-60662ebe5dd0" xlink:to="loc_us-gaap_Depreciation_93aa7a35-796d-402c-8f7a-97a211b712db" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInPrepaidExpense_b4929b55-85fb-4484-8ef1-efa90c53ebbe" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInPrepaidExpense"/>
    <link:calculationArc order="11" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_2b40e1a1-ac43-4012-b831-60662ebe5dd0" xlink:to="loc_us-gaap_IncreaseDecreaseInPrepaidExpense_b4929b55-85fb-4484-8ef1-efa90c53ebbe" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationContingentConsiderationArrangementsChangeInAmountOfContingentConsiderationLiability1_862aef41-9958-41cd-9846-31c0bf8cbaab" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationContingentConsiderationArrangementsChangeInAmountOfContingentConsiderationLiability1"/>
    <link:calculationArc order="12" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_2b40e1a1-ac43-4012-b831-60662ebe5dd0" xlink:to="loc_us-gaap_BusinessCombinationContingentConsiderationArrangementsChangeInAmountOfContingentConsiderationLiability1_862aef41-9958-41cd-9846-31c0bf8cbaab" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccruedLiabilities_2019fdf4-38a7-4bb0-b6d5-0131e14d25ce" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInAccruedLiabilities"/>
    <link:calculationArc order="13" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_2b40e1a1-ac43-4012-b831-60662ebe5dd0" xlink:to="loc_us-gaap_IncreaseDecreaseInAccruedLiabilities_2019fdf4-38a7-4bb0-b6d5-0131e14d25ce" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GainLossOnSaleOfPropertyPlantEquipment_4828dd5a-cbbb-40c9-b8dd-7f0cac74b9a2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_GainLossOnSaleOfPropertyPlantEquipment"/>
    <link:calculationArc order="14" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_2b40e1a1-ac43-4012-b831-60662ebe5dd0" xlink:to="loc_us-gaap_GainLossOnSaleOfPropertyPlantEquipment_4828dd5a-cbbb-40c9-b8dd-7f0cac74b9a2" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInOperatingLeaseLiability_e57d700b-7d33-4773-b5f5-0804fa0d9a01" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInOperatingLeaseLiability"/>
    <link:calculationArc order="15" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_2b40e1a1-ac43-4012-b831-60662ebe5dd0" xlink:to="loc_us-gaap_IncreaseDecreaseInOperatingLeaseLiability_e57d700b-7d33-4773-b5f5-0804fa0d9a01" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsPayable_37bc437e-8a23-4eac-9ff6-f8cb9f2b5d5a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInAccountsPayable"/>
    <link:calculationArc order="16" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_2b40e1a1-ac43-4012-b831-60662ebe5dd0" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsPayable_37bc437e-8a23-4eac-9ff6-f8cb9f2b5d5a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_a9667ed9-d609-4c1d-9f7f-1c08ee9c6fbb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_27e4b1a9-0fd5-4109-801b-dce27f1ae9cf" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_a9667ed9-d609-4c1d-9f7f-1c08ee9c6fbb" xlink:to="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_27e4b1a9-0fd5-4109-801b-dce27f1ae9cf" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_811becb5-550e-4819-8fa8-178091aaf622" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivities"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_a9667ed9-d609-4c1d-9f7f-1c08ee9c6fbb" xlink:to="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_811becb5-550e-4819-8fa8-178091aaf622" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_0883a3a4-eb68-41b8-a1e8-b56352b1eddd" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivities"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_a9667ed9-d609-4c1d-9f7f-1c08ee9c6fbb" xlink:to="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_0883a3a4-eb68-41b8-a1e8-b56352b1eddd" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.humacyte.com/role/SummaryofSignificantAccountingPoliciesRestrictedCashDetails" xlink:type="simple" xlink:href="huma-20241231.xsd#SummaryofSignificantAccountingPoliciesRestrictedCashDetails"/>
  <link:calculationLink xlink:role="http://www.humacyte.com/role/SummaryofSignificantAccountingPoliciesRestrictedCashDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_0295d1bf-a1d7-4267-9df5-6158ca14319c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCashNoncurrent_aefa58d9-f421-4241-89e2-72c4882d6cdc" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RestrictedCashNoncurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_0295d1bf-a1d7-4267-9df5-6158ca14319c" xlink:to="loc_us-gaap_RestrictedCashNoncurrent_aefa58d9-f421-4241-89e2-72c4882d6cdc" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_882d7733-578b-431c-a622-af9e473f9d39" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_0295d1bf-a1d7-4267-9df5-6158ca14319c" xlink:to="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_882d7733-578b-431c-a622-af9e473f9d39" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCashCurrent_a301bc58-38ab-4eed-98d9-e6182c4baa8c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RestrictedCashCurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_0295d1bf-a1d7-4267-9df5-6158ca14319c" xlink:to="loc_us-gaap_RestrictedCashCurrent_a301bc58-38ab-4eed-98d9-e6182c4baa8c" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.humacyte.com/role/FairValueMeasurementsAssetsandLiabilitiesMeasuredatFairValueonRecurringBasisDetails" xlink:type="simple" xlink:href="huma-20241231.xsd#FairValueMeasurementsAssetsandLiabilitiesMeasuredatFairValueonRecurringBasisDetails"/>
  <link:calculationLink xlink:role="http://www.humacyte.com/role/FairValueMeasurementsAssetsandLiabilitiesMeasuredatFairValueonRecurringBasisDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsFairValueDisclosure_bda5c02d-a9d0-42d9-a168-1bd3d19df9f4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AssetsFairValueDisclosure"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeAssets_21ff99cb-fd75-4c41-84b8-b5b4ad4bc13a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DerivativeAssets"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsFairValueDisclosure_bda5c02d-a9d0-42d9-a168-1bd3d19df9f4" xlink:to="loc_us-gaap_DerivativeAssets_21ff99cb-fd75-4c41-84b8-b5b4ad4bc13a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_c1e2aa2c-0d9d-4fc0-a355-10d8deec11a2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashAndCashEquivalentsFairValueDisclosure"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsFairValueDisclosure_bda5c02d-a9d0-42d9-a168-1bd3d19df9f4" xlink:to="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_c1e2aa2c-0d9d-4fc0-a355-10d8deec11a2" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialLiabilitiesFairValueDisclosure_ba8bc24d-988e-4522-bb83-cd22439c7a1e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FinancialLiabilitiesFairValueDisclosure"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WarrantsAndRightsOutstanding_0ea27a0c-2006-4898-99b8-994c2b054055" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_WarrantsAndRightsOutstanding"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_FinancialLiabilitiesFairValueDisclosure_ba8bc24d-988e-4522-bb83-cd22439c7a1e" xlink:to="loc_us-gaap_WarrantsAndRightsOutstanding_0ea27a0c-2006-4898-99b8-994c2b054055" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeLiabilities_019a598d-b321-4f76-a73e-d18529b9414c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DerivativeLiabilities"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_FinancialLiabilitiesFairValueDisclosure_ba8bc24d-988e-4522-bb83-cd22439c7a1e" xlink:to="loc_us-gaap_DerivativeLiabilities_019a598d-b321-4f76-a73e-d18529b9414c" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationContingentConsiderationLiability_33967c0d-fec6-478f-b021-9abaf34664ee" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationContingentConsiderationLiability"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_FinancialLiabilitiesFairValueDisclosure_ba8bc24d-988e-4522-bb83-cd22439c7a1e" xlink:to="loc_us-gaap_BusinessCombinationContingentConsiderationLiability_33967c0d-fec6-478f-b021-9abaf34664ee" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsFairValueDisclosure_aceda94b-95cc-4806-92ef-efe5d153926a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AssetsFairValueDisclosure"/>
    <link:calculationArc order="4" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_FinancialLiabilitiesFairValueDisclosure_ba8bc24d-988e-4522-bb83-cd22439c7a1e" xlink:to="loc_us-gaap_AssetsFairValueDisclosure_aceda94b-95cc-4806-92ef-efe5d153926a" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.humacyte.com/role/PropertyandEquipmentNetDetails" xlink:type="simple" xlink:href="huma-20241231.xsd#PropertyandEquipmentNetDetails"/>
  <link:calculationLink xlink:role="http://www.humacyte.com/role/PropertyandEquipmentNetDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_77af823b-b555-4e69-a2f5-b7801aa9e84a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentGross_383fedc5-6f9f-46df-b5d2-686284d6a1ec" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PropertyPlantAndEquipmentGross"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentNet_77af823b-b555-4e69-a2f5-b7801aa9e84a" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentGross_383fedc5-6f9f-46df-b5d2-686284d6a1ec" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_1bb376b9-33de-43b0-84fc-1981078cc043" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentNet_77af823b-b555-4e69-a2f5-b7801aa9e84a" xlink:to="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_1bb376b9-33de-43b0-84fc-1981078cc043" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.humacyte.com/role/AccruedExpensesDetails" xlink:type="simple" xlink:href="huma-20241231.xsd#AccruedExpensesDetails"/>
  <link:calculationLink xlink:role="http://www.humacyte.com/role/AccruedExpensesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccruedLiabilitiesCurrent_68ab493e-acfe-4fec-9c88-c36bebe6f6a1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccruedLiabilitiesCurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_huma_AccruedExternalResearchDevelopmentAndManufacturingCostsCurrent_1d2021e9-6a25-4c8e-a2a9-746e2a71ed16" xlink:href="huma-20241231.xsd#huma_AccruedExternalResearchDevelopmentAndManufacturingCostsCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AccruedLiabilitiesCurrent_68ab493e-acfe-4fec-9c88-c36bebe6f6a1" xlink:to="loc_huma_AccruedExternalResearchDevelopmentAndManufacturingCostsCurrent_1d2021e9-6a25-4c8e-a2a9-746e2a71ed16" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeRelatedLiabilitiesCurrent_ba2d097b-b1e5-46e3-9a9d-3d6bc93d4a7e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EmployeeRelatedLiabilitiesCurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AccruedLiabilitiesCurrent_68ab493e-acfe-4fec-9c88-c36bebe6f6a1" xlink:to="loc_us-gaap_EmployeeRelatedLiabilitiesCurrent_ba2d097b-b1e5-46e3-9a9d-3d6bc93d4a7e" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccruedProfessionalFeesCurrent_750327da-5b91-4089-973f-69586429bd64" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccruedProfessionalFeesCurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AccruedLiabilitiesCurrent_68ab493e-acfe-4fec-9c88-c36bebe6f6a1" xlink:to="loc_us-gaap_AccruedProfessionalFeesCurrent_750327da-5b91-4089-973f-69586429bd64" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.humacyte.com/role/LeasesTotalLeaseCostDetails" xlink:type="simple" xlink:href="huma-20241231.xsd#LeasesTotalLeaseCostDetails"/>
  <link:calculationLink xlink:role="http://www.humacyte.com/role/LeasesTotalLeaseCostDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LeaseCost_c16a47f9-70fb-43d2-82be-bed372bfdbd0" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LeaseCost"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseCost_07a90c62-abc5-4e8a-a076-e6df7f3e6d36" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingLeaseCost"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LeaseCost_c16a47f9-70fb-43d2-82be-bed372bfdbd0" xlink:to="loc_us-gaap_OperatingLeaseCost_07a90c62-abc5-4e8a-a076-e6df7f3e6d36" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_huma_FinanceLeaseCost_8e1cb59e-1bdb-4761-a714-59f36d7fb8be" xlink:href="huma-20241231.xsd#huma_FinanceLeaseCost"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LeaseCost_c16a47f9-70fb-43d2-82be-bed372bfdbd0" xlink:to="loc_huma_FinanceLeaseCost_8e1cb59e-1bdb-4761-a714-59f36d7fb8be" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_huma_FinanceLeaseCost_526ee5e5-21fd-4fb2-9a58-558746e5f984" xlink:href="huma-20241231.xsd#huma_FinanceLeaseCost"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinanceLeaseInterestExpense_9b718576-1785-447a-9fa7-92499ebe868f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FinanceLeaseInterestExpense"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_huma_FinanceLeaseCost_526ee5e5-21fd-4fb2-9a58-558746e5f984" xlink:to="loc_us-gaap_FinanceLeaseInterestExpense_9b718576-1785-447a-9fa7-92499ebe868f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinanceLeaseRightOfUseAssetAmortization_38af5977-e19c-48a3-a48a-cf788a5dc66a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FinanceLeaseRightOfUseAssetAmortization"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_huma_FinanceLeaseCost_526ee5e5-21fd-4fb2-9a58-558746e5f984" xlink:to="loc_us-gaap_FinanceLeaseRightOfUseAssetAmortization_38af5977-e19c-48a3-a48a-cf788a5dc66a" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.humacyte.com/role/LeasesMaturitiesofLeaseLiabilitiesDetails" xlink:type="simple" xlink:href="huma-20241231.xsd#LeasesMaturitiesofLeaseLiabilitiesDetails"/>
  <link:calculationLink xlink:role="http://www.humacyte.com/role/LeasesMaturitiesofLeaseLiabilitiesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinanceLeaseLiabilityPaymentsDue_443b73ab-3e74-4862-b83d-cd0f2f27bb12" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FinanceLeaseLiabilityPaymentsDue"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinanceLeaseLiabilityPaymentsDueYearFive_93c63c11-4b16-4eed-b8fc-ebde702d6675" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FinanceLeaseLiabilityPaymentsDueYearFive"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_FinanceLeaseLiabilityPaymentsDue_443b73ab-3e74-4862-b83d-cd0f2f27bb12" xlink:to="loc_us-gaap_FinanceLeaseLiabilityPaymentsDueYearFive_93c63c11-4b16-4eed-b8fc-ebde702d6675" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinanceLeaseLiabilityPaymentsDueNextTwelveMonths_6adea107-c655-468e-ab18-d09a2a297829" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FinanceLeaseLiabilityPaymentsDueNextTwelveMonths"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_FinanceLeaseLiabilityPaymentsDue_443b73ab-3e74-4862-b83d-cd0f2f27bb12" xlink:to="loc_us-gaap_FinanceLeaseLiabilityPaymentsDueNextTwelveMonths_6adea107-c655-468e-ab18-d09a2a297829" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinanceLeaseLiabilityPaymentsDueYearThree_e84ed4f7-5103-479e-976a-66be857957c5" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FinanceLeaseLiabilityPaymentsDueYearThree"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_FinanceLeaseLiabilityPaymentsDue_443b73ab-3e74-4862-b83d-cd0f2f27bb12" xlink:to="loc_us-gaap_FinanceLeaseLiabilityPaymentsDueYearThree_e84ed4f7-5103-479e-976a-66be857957c5" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinanceLeaseLiabilityPaymentsDueAfterYearFive_6f26d44f-ed16-4fa6-bab4-d6d0bffad47a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FinanceLeaseLiabilityPaymentsDueAfterYearFive"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_FinanceLeaseLiabilityPaymentsDue_443b73ab-3e74-4862-b83d-cd0f2f27bb12" xlink:to="loc_us-gaap_FinanceLeaseLiabilityPaymentsDueAfterYearFive_6f26d44f-ed16-4fa6-bab4-d6d0bffad47a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinanceLeaseLiabilityPaymentsDueYearTwo_4ba3da67-7fec-4b01-8d7a-d7b1188e4e4c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FinanceLeaseLiabilityPaymentsDueYearTwo"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_FinanceLeaseLiabilityPaymentsDue_443b73ab-3e74-4862-b83d-cd0f2f27bb12" xlink:to="loc_us-gaap_FinanceLeaseLiabilityPaymentsDueYearTwo_4ba3da67-7fec-4b01-8d7a-d7b1188e4e4c" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinanceLeaseLiabilityPaymentsDueYearFour_afaaa7e5-685e-426d-b386-531f0e87d2b1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FinanceLeaseLiabilityPaymentsDueYearFour"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_FinanceLeaseLiabilityPaymentsDue_443b73ab-3e74-4862-b83d-cd0f2f27bb12" xlink:to="loc_us-gaap_FinanceLeaseLiabilityPaymentsDueYearFour_afaaa7e5-685e-426d-b386-531f0e87d2b1" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_4ee58071-581d-4559-907a-e33b6c142384" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityUndiscountedExcessAmount_ddba9f60-051e-41b4-b4af-4ab5619e61bb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LesseeOperatingLeaseLiabilityUndiscountedExcessAmount"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_4ee58071-581d-4559-907a-e33b6c142384" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityUndiscountedExcessAmount_ddba9f60-051e-41b4-b4af-4ab5619e61bb" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiability_c96f529b-b22d-41ed-a53d-250df6e77b3c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingLeaseLiability"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_4ee58071-581d-4559-907a-e33b6c142384" xlink:to="loc_us-gaap_OperatingLeaseLiability_c96f529b-b22d-41ed-a53d-250df6e77b3c" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.humacyte.com/role/LeasesMaturitiesofLeaseLiabilitiesDetails_1" xlink:type="simple" xlink:href="huma-20241231.xsd#LeasesMaturitiesofLeaseLiabilitiesDetails_1"/>
  <link:calculationLink xlink:role="http://www.humacyte.com/role/LeasesMaturitiesofLeaseLiabilitiesDetails_1" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinanceLeaseLiabilityPaymentsDue_cd520fc8-0c1f-46e4-b803-5d3396e60e8a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FinanceLeaseLiabilityPaymentsDue"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinanceLeaseLiabilityUndiscountedExcessAmount_220f1cba-70bd-48c8-8ba1-ad2758021048" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FinanceLeaseLiabilityUndiscountedExcessAmount"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_FinanceLeaseLiabilityPaymentsDue_cd520fc8-0c1f-46e4-b803-5d3396e60e8a" xlink:to="loc_us-gaap_FinanceLeaseLiabilityUndiscountedExcessAmount_220f1cba-70bd-48c8-8ba1-ad2758021048" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinanceLeaseLiability_8ff8417f-a871-4291-b9b8-9946c480f0fc" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FinanceLeaseLiability"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_FinanceLeaseLiabilityPaymentsDue_cd520fc8-0c1f-46e4-b803-5d3396e60e8a" xlink:to="loc_us-gaap_FinanceLeaseLiability_8ff8417f-a871-4291-b9b8-9946c480f0fc" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_b52f3cca-cbea-4d82-a647-50f0de7d8b9a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFour_6dde2e4d-cbdd-4369-b1df-c954cfabc98e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFour"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_b52f3cca-cbea-4d82-a647-50f0de7d8b9a" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFour_6dde2e4d-cbdd-4369-b1df-c954cfabc98e" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFive_e6400313-6484-424d-af5e-188b1ca7614b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFive"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_b52f3cca-cbea-4d82-a647-50f0de7d8b9a" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFive_e6400313-6484-424d-af5e-188b1ca7614b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths_e40ee7da-de84-4608-bb90-7c33b5b65ff3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_b52f3cca-cbea-4d82-a647-50f0de7d8b9a" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths_e40ee7da-de84-4608-bb90-7c33b5b65ff3" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueAfterYearFive_13893a9a-a66c-4283-a41b-5e829603e26c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueAfterYearFive"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_b52f3cca-cbea-4d82-a647-50f0de7d8b9a" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueAfterYearFive_13893a9a-a66c-4283-a41b-5e829603e26c" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo_a98d0c57-b2f2-4b89-b304-d7f02d1213f0" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_b52f3cca-cbea-4d82-a647-50f0de7d8b9a" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo_a98d0c57-b2f2-4b89-b304-d7f02d1213f0" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearThree_2e7acd09-b566-4039-ad4c-49b64630fb8f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearThree"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_b52f3cca-cbea-4d82-a647-50f0de7d8b9a" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearThree_2e7acd09-b566-4039-ad4c-49b64630fb8f" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.humacyte.com/role/IncomeTaxesDeferredTaxAssetsandDeferredTaxLiabilitiesDetails" xlink:type="simple" xlink:href="huma-20241231.xsd#IncomeTaxesDeferredTaxAssetsandDeferredTaxLiabilitiesDetails"/>
  <link:calculationLink xlink:role="http://www.humacyte.com/role/IncomeTaxesDeferredTaxAssetsandDeferredTaxLiabilitiesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredIncomeTaxLiabilitiesNet_f417a24c-fc82-40a9-8ff0-7a2236aff730" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DeferredIncomeTaxLiabilitiesNet"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxLiabilitiesPropertyPlantAndEquipment_67bb6dd2-1bc0-49f8-8edb-71ae21d321de" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DeferredTaxLiabilitiesPropertyPlantAndEquipment"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DeferredIncomeTaxLiabilitiesNet_f417a24c-fc82-40a9-8ff0-7a2236aff730" xlink:to="loc_us-gaap_DeferredTaxLiabilitiesPropertyPlantAndEquipment_67bb6dd2-1bc0-49f8-8edb-71ae21d321de" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxLiabilitiesLeasingArrangements_9054a322-f98b-49b8-b7e1-a982605b6b5d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DeferredTaxLiabilitiesLeasingArrangements"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DeferredIncomeTaxLiabilitiesNet_f417a24c-fc82-40a9-8ff0-7a2236aff730" xlink:to="loc_us-gaap_DeferredTaxLiabilitiesLeasingArrangements_9054a322-f98b-49b8-b7e1-a982605b6b5d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxAssetsNet_5ed6515c-db9e-478c-b0ea-b274e106595c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DeferredTaxAssetsNet"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxAssetsGross_cee03df8-da4f-42c5-95ab-6df5720cd511" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DeferredTaxAssetsGross"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DeferredTaxAssetsNet_5ed6515c-db9e-478c-b0ea-b274e106595c" xlink:to="loc_us-gaap_DeferredTaxAssetsGross_cee03df8-da4f-42c5-95ab-6df5720cd511" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxAssetsValuationAllowance_c22c5f76-d346-40a0-bf5a-bbfdab248051" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DeferredTaxAssetsValuationAllowance"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DeferredTaxAssetsNet_5ed6515c-db9e-478c-b0ea-b274e106595c" xlink:to="loc_us-gaap_DeferredTaxAssetsValuationAllowance_c22c5f76-d346-40a0-bf5a-bbfdab248051" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxAssetsGross_d195b433-c443-4c11-84a5-b6048bd8294b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DeferredTaxAssetsGross"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxAssetsOperatingLossCarryforwards_e38085f1-a608-43f4-82aa-985c79979b38" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DeferredTaxAssetsOperatingLossCarryforwards"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DeferredTaxAssetsGross_d195b433-c443-4c11-84a5-b6048bd8294b" xlink:to="loc_us-gaap_DeferredTaxAssetsOperatingLossCarryforwards_e38085f1-a608-43f4-82aa-985c79979b38" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxAssetsInProcessResearchAndDevelopment_61fc0c04-eabf-4cad-a985-08c55050934c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DeferredTaxAssetsInProcessResearchAndDevelopment"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DeferredTaxAssetsGross_d195b433-c443-4c11-84a5-b6048bd8294b" xlink:to="loc_us-gaap_DeferredTaxAssetsInProcessResearchAndDevelopment_61fc0c04-eabf-4cad-a985-08c55050934c" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxAssetsTaxCreditCarryforwardsResearch_8d7eadfc-eae4-4e5c-b4f8-1d79568d7156" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DeferredTaxAssetsTaxCreditCarryforwardsResearch"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DeferredTaxAssetsGross_d195b433-c443-4c11-84a5-b6048bd8294b" xlink:to="loc_us-gaap_DeferredTaxAssetsTaxCreditCarryforwardsResearch_8d7eadfc-eae4-4e5c-b4f8-1d79568d7156" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxAssetsTaxDeferredExpenseCompensationAndBenefitsShareBasedCompensationCost_a1c798c5-1e34-4e48-937e-e166adf785e7" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DeferredTaxAssetsTaxDeferredExpenseCompensationAndBenefitsShareBasedCompensationCost"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DeferredTaxAssetsGross_d195b433-c443-4c11-84a5-b6048bd8294b" xlink:to="loc_us-gaap_DeferredTaxAssetsTaxDeferredExpenseCompensationAndBenefitsShareBasedCompensationCost_a1c798c5-1e34-4e48-937e-e166adf785e7" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_huma_DeferredTaxAssetsRightOfUseLiabilities_86442166-31ee-4b81-9096-5595a79d76dd" xlink:href="huma-20241231.xsd#huma_DeferredTaxAssetsRightOfUseLiabilities"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DeferredTaxAssetsGross_d195b433-c443-4c11-84a5-b6048bd8294b" xlink:to="loc_huma_DeferredTaxAssetsRightOfUseLiabilities_86442166-31ee-4b81-9096-5595a79d76dd" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxAssetsTaxDeferredExpenseReservesAndAccrualsAccruedLiabilities_c8029de0-fcb4-4630-984a-a5738c33b690" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DeferredTaxAssetsTaxDeferredExpenseReservesAndAccrualsAccruedLiabilities"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DeferredTaxAssetsGross_d195b433-c443-4c11-84a5-b6048bd8294b" xlink:to="loc_us-gaap_DeferredTaxAssetsTaxDeferredExpenseReservesAndAccrualsAccruedLiabilities_c8029de0-fcb4-4630-984a-a5738c33b690" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxAssetsOther_cddf74b6-3354-4708-9642-4948d62c58aa" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DeferredTaxAssetsOther"/>
    <link:calculationArc order="7" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DeferredTaxAssetsGross_d195b433-c443-4c11-84a5-b6048bd8294b" xlink:to="loc_us-gaap_DeferredTaxAssetsOther_cddf74b6-3354-4708-9642-4948d62c58aa" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxAssetsLiabilitiesNet_40588ce9-6027-4e79-b45a-73fbd3890ea0" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DeferredTaxAssetsLiabilitiesNet"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxAssetsNet_527ee724-0db5-4b98-85bf-7c959b3fd8cb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DeferredTaxAssetsNet"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DeferredTaxAssetsLiabilitiesNet_40588ce9-6027-4e79-b45a-73fbd3890ea0" xlink:to="loc_us-gaap_DeferredTaxAssetsNet_527ee724-0db5-4b98-85bf-7c959b3fd8cb" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredIncomeTaxLiabilitiesNet_b04ca9bc-3449-45e7-8b2a-5c7edf9196ae" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DeferredIncomeTaxLiabilitiesNet"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DeferredTaxAssetsLiabilitiesNet_40588ce9-6027-4e79-b45a-73fbd3890ea0" xlink:to="loc_us-gaap_DeferredIncomeTaxLiabilitiesNet_b04ca9bc-3449-45e7-8b2a-5c7edf9196ae" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.humacyte.com/role/IncomeTaxesEffectiveIncomeTaxRateReconciliationDetails" xlink:type="simple" xlink:href="huma-20241231.xsd#IncomeTaxesEffectiveIncomeTaxRateReconciliationDetails"/>
  <link:calculationLink xlink:role="http://www.humacyte.com/role/IncomeTaxesEffectiveIncomeTaxRateReconciliationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EffectiveIncomeTaxRateContinuingOperations_ee66680f-f596-45fd-84a4-22d1946b667c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EffectiveIncomeTaxRateContinuingOperations"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EffectiveIncomeTaxRateReconciliationChangeInDeferredTaxAssetsValuationAllowance_e5a47e68-800a-418e-a85e-caf32413185c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EffectiveIncomeTaxRateReconciliationChangeInDeferredTaxAssetsValuationAllowance"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_EffectiveIncomeTaxRateContinuingOperations_ee66680f-f596-45fd-84a4-22d1946b667c" xlink:to="loc_us-gaap_EffectiveIncomeTaxRateReconciliationChangeInDeferredTaxAssetsValuationAllowance_e5a47e68-800a-418e-a85e-caf32413185c" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EffectiveIncomeTaxRateReconciliationOtherAdjustments_412f8fd4-6e34-49d8-9c70-eb404e6d4ff2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EffectiveIncomeTaxRateReconciliationOtherAdjustments"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_EffectiveIncomeTaxRateContinuingOperations_ee66680f-f596-45fd-84a4-22d1946b667c" xlink:to="loc_us-gaap_EffectiveIncomeTaxRateReconciliationOtherAdjustments_412f8fd4-6e34-49d8-9c70-eb404e6d4ff2" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EffectiveIncomeTaxRateReconciliationChangeInEnactedTaxRate_889ce321-eaec-4f36-9f8f-521dff9f25ad" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EffectiveIncomeTaxRateReconciliationChangeInEnactedTaxRate"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_EffectiveIncomeTaxRateContinuingOperations_ee66680f-f596-45fd-84a4-22d1946b667c" xlink:to="loc_us-gaap_EffectiveIncomeTaxRateReconciliationChangeInEnactedTaxRate_889ce321-eaec-4f36-9f8f-521dff9f25ad" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EffectiveIncomeTaxRateReconciliationNondeductibleExpenseOther_625e05ed-75aa-4bff-9555-d4e6078b2952" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EffectiveIncomeTaxRateReconciliationNondeductibleExpenseOther"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_EffectiveIncomeTaxRateContinuingOperations_ee66680f-f596-45fd-84a4-22d1946b667c" xlink:to="loc_us-gaap_EffectiveIncomeTaxRateReconciliationNondeductibleExpenseOther_625e05ed-75aa-4bff-9555-d4e6078b2952" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EffectiveIncomeTaxRateReconciliationTaxCredits_d86b3bbe-2e67-466e-bd5a-ea90a0d1ceac" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EffectiveIncomeTaxRateReconciliationTaxCredits"/>
    <link:calculationArc order="5" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_EffectiveIncomeTaxRateContinuingOperations_ee66680f-f596-45fd-84a4-22d1946b667c" xlink:to="loc_us-gaap_EffectiveIncomeTaxRateReconciliationTaxCredits_d86b3bbe-2e67-466e-bd5a-ea90a0d1ceac" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EffectiveIncomeTaxRateReconciliationStateAndLocalIncomeTaxes_a2f00e20-121c-416b-8d2a-b62a3fda1999" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EffectiveIncomeTaxRateReconciliationStateAndLocalIncomeTaxes"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_EffectiveIncomeTaxRateContinuingOperations_ee66680f-f596-45fd-84a4-22d1946b667c" xlink:to="loc_us-gaap_EffectiveIncomeTaxRateReconciliationStateAndLocalIncomeTaxes_a2f00e20-121c-416b-8d2a-b62a3fda1999" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EffectiveIncomeTaxRateReconciliationAtFederalStatutoryIncomeTaxRate_5907c1e4-b3e3-47c7-87a6-cafb3f86399b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EffectiveIncomeTaxRateReconciliationAtFederalStatutoryIncomeTaxRate"/>
    <link:calculationArc order="7" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_EffectiveIncomeTaxRateContinuingOperations_ee66680f-f596-45fd-84a4-22d1946b667c" xlink:to="loc_us-gaap_EffectiveIncomeTaxRateReconciliationAtFederalStatutoryIncomeTaxRate_5907c1e4-b3e3-47c7-87a6-cafb3f86399b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxExpenseBenefit_937f9f95-e827-4e35-9ef7-b57be9ef97de" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncomeTaxExpenseBenefit"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxReconciliationChangeInDeferredTaxAssetsValuationAllowance_642b1a08-8935-4f31-bbdb-9325a77c23be" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncomeTaxReconciliationChangeInDeferredTaxAssetsValuationAllowance"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeTaxExpenseBenefit_937f9f95-e827-4e35-9ef7-b57be9ef97de" xlink:to="loc_us-gaap_IncomeTaxReconciliationChangeInDeferredTaxAssetsValuationAllowance_642b1a08-8935-4f31-bbdb-9325a77c23be" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxReconciliationOtherAdjustments_7dbc21e8-6af2-43d0-b01e-172c7ee84125" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncomeTaxReconciliationOtherAdjustments"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeTaxExpenseBenefit_937f9f95-e827-4e35-9ef7-b57be9ef97de" xlink:to="loc_us-gaap_IncomeTaxReconciliationOtherAdjustments_7dbc21e8-6af2-43d0-b01e-172c7ee84125" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxReconciliationChangeInEnactedTaxRate_6d71b298-ca23-45d3-8ad0-6feb453afa2f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncomeTaxReconciliationChangeInEnactedTaxRate"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeTaxExpenseBenefit_937f9f95-e827-4e35-9ef7-b57be9ef97de" xlink:to="loc_us-gaap_IncomeTaxReconciliationChangeInEnactedTaxRate_6d71b298-ca23-45d3-8ad0-6feb453afa2f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxReconciliationNondeductibleExpenseOther_db49b55b-f190-4692-86e8-ccd291a475ca" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncomeTaxReconciliationNondeductibleExpenseOther"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeTaxExpenseBenefit_937f9f95-e827-4e35-9ef7-b57be9ef97de" xlink:to="loc_us-gaap_IncomeTaxReconciliationNondeductibleExpenseOther_db49b55b-f190-4692-86e8-ccd291a475ca" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxReconciliationTaxCredits_81e4a15b-a8d1-4978-9549-eff7ebd47488" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncomeTaxReconciliationTaxCredits"/>
    <link:calculationArc order="5" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeTaxExpenseBenefit_937f9f95-e827-4e35-9ef7-b57be9ef97de" xlink:to="loc_us-gaap_IncomeTaxReconciliationTaxCredits_81e4a15b-a8d1-4978-9549-eff7ebd47488" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxReconciliationStateAndLocalIncomeTaxes_bbeaa768-6205-46de-838b-6f609d0e6832" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncomeTaxReconciliationStateAndLocalIncomeTaxes"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeTaxExpenseBenefit_937f9f95-e827-4e35-9ef7-b57be9ef97de" xlink:to="loc_us-gaap_IncomeTaxReconciliationStateAndLocalIncomeTaxes_bbeaa768-6205-46de-838b-6f609d0e6832" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxReconciliationIncomeTaxExpenseBenefitAtFederalStatutoryIncomeTaxRate_f6bc14d8-637b-474c-ad6a-e034135d246d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncomeTaxReconciliationIncomeTaxExpenseBenefitAtFederalStatutoryIncomeTaxRate"/>
    <link:calculationArc order="7" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeTaxExpenseBenefit_937f9f95-e827-4e35-9ef7-b57be9ef97de" xlink:to="loc_us-gaap_IncomeTaxReconciliationIncomeTaxExpenseBenefitAtFederalStatutoryIncomeTaxRate_f6bc14d8-637b-474c-ad6a-e034135d246d" xlink:type="arc"/>
  </link:calculationLink>
</link:linkbase>
